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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- 711441 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FMRP Recombinant Polyclonal Antibody (5HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Cat, Pig and Sheep
- Antibody clone number
- 5HCLC
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, SHSY-5Y cells were fixed and permeabilized for detection of endogenous FMR1 using Anti- FMR1 Recombinant Rabbit Polyclonal Antibody (Product # 711441, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of FMR1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of FMR1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
