TA504293

antibody from OriGene

Targeting: FMR1 FMRP, FRAXA, MGC87458, POF, POF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504293

Provider

OriGene

Proper citation

OriGene Cat#TA504293, RRID:AB_11125303

Product name

FMR1 (FMRP ) mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Antibody type

Monoclonal

Description

FMR1 (FMRP ) mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

FMR1

Isotype

IgG

Antibody clone number

OTI2C3

Vial size

100 µl

Concentration

0.96 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMR1 (RC222699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMR1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FMR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504293)

Validation comment

IHC