MA5-15499

antibody from Invitrogen Antibodies

Targeting: FMR1 FMRP, FRAXA, MGC87458, POF, POF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15499

Provider

Invitrogen Antibodies

Product name

FMRP Monoclonal Antibody (4G9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15499 targets FMR1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15499 immunogen is purified recombinant fragment of human FMR1 expressed in E. Coli. MA5-15499 detects FMR1 which has a predicted molecular weight of approximately 71kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4G9

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion.

Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ
eLife 2019 Mar 29;8

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New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.

Das R, Schwintzer L, Vinopal S, Aguado Roca E, Sylvester M, Oprisoreanu AM, Schoch S, Bradke F, Broemer M
Journal of cell science 2019 Jun 17;132(12)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), SH-SY5Y (Lane 2), Jurkat (Lane 3), K-562 (Lane 4), U-87 MG (Lane 5) and HeLa (Lane 6). The blot was probed with Anti-FMRP Monoclonal Antibody (Product # MA5-15499, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 71 kDa band corresponding to FMRP was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of FMRP was achieved by transfecting SH-SY5Y with FMRP specific siRNAs (Silencer® select Product # s5317, s5316 ). Western blot analysis (Fig. a) was performed using whole cell extracts from the FMRP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FMRP Monoclonal Antibody (Product # MA5-15499, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FMRP.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using FMR1 monoclonal antibody (Product # MA5-15499) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of FMRP was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FMRP Mouse Monoclonal Antibody (Product # MA5-15499) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human brain tissues using FMR1 monoclonal antibody (Product # MA5-15499) followed with DAB staining

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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