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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FMRP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-27353 targets FMRP in IF and WB applications and shows reactivity with Human samples. The PA5-27353 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 181 of Human FMRP.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.26 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FMRP using 30µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FMRP polyclonal antibody (Product # PA5-27353) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FMRP in paraformaldehyde-fixed HeLa cells using a FMRP polyclonal antibody (Product # PA5-27353) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FMRP in the cytosol of paraffin-embedded hepatoma using a FMRP polyclonal antibody (Product # PA5-27353) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FMRP in the cytosol of paraffin-embedded mouse liver using a FMRP polyclonal antibody (Product # PA5-27353) at a dilution of 1:500.
