PA5-88644
antibody from Invitrogen Antibodies
Targeting: PRPF19
hPSO4, NMP200, PRP19, PSO4, SNEV, UBOX4
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-88644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRP19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AREALATLKP QAGLIVPQAV PSSQPSVVGA GEPMDLGELV GMTPEIIQKL QDKATVLTTE RKKRGKTVPE ELVKPEELSK YRQVASHVGL HSASIPGILA LDLCPSDTNK ILTGGADKNV VVFDKSSEQI LATLKGHTKK VTSVVFHPSQ DLVFSASPDA TIRIWSVPNA SCVQVVRAHE SAVTGLSLHA TGDYLLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAFSENGYY LATAADDSSV; Positive Samples: HepG2, Jurkat, HeLa, Mouse heart, Rat brain; Cellular Location: Cytoplasm, Lipid droplet, Nucleus, cytoskeleton, nucleoplasm, spindle
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.54 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PRP19 in H9C2 cells. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PRP19 in L929 cells. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PRP19 in U2OS cells. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PRP19 in 200 μg extracts of Jurkat cells. Samples were precipitated with 3 μg PRP19 Polyclonal antibody (Product # PA5-88644). Western blot was performed from the immunoprecipitate using PRP19 Polyclonal antibody (Product # PA5-88644) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRP19 in paraffin-embedded rat liver. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRP19 in paraffin-embedded human lung cancer. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRP19 in paraffin-embedded human stomach. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRP19 in paraffin-embedded mouse brain. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of PRP19 was performed in 200 µg extracts of Jurkat cells using PRP19 Polyclonal Antibody (Product # PA5-88644). Western blot was performed from the immunoprecipitate using PRP19 Polyclonal Antibody.