Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28573 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FRK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (84%), Rat (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FRK Polyclonal Antibody (Product # PA5-28573). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. FRK Polyclonal Antibody . FRK Polyclonal Antibody (Product # PA5-28573) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FRK Polyclonal Antibody detects FRK protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min. Green: FRK protein stained by FRK Polyclonal Antibody (Product # PA5-28573) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using FRK (Product # PA5-28573) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
