Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- 720278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GHSR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on tissue and membrane enriched cell extracts (30 µg lysate) of A549 (Lane 1), U-87 MG (Lane 2), Hep G2 (Lane 3), Mouse liver (Lane 4), Rat liver (Lane 5), SH-SY5Y (Lane 6), HEL 92.1.7 (Lane 7) and NTERA-2 (Lane 8). The blots were probed with Growth hormone secretagogue receptor 1a/GHSR1a Rabbit Polyclonal antibody (Product # 720278, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~48 kDa band corresponding to Growth hormone secretagogue receptor 1a/GHSR1a was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Knockdown of GHSR was achieved by transfecting A549 cells with GHSR specific siRNAs (Silencer® select Product # s194432, s194431). Western blot analysis (Fig. a) was performed using whole cell extracts from the GHSR knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GHSR Polyclonal Antibody (Product # 720278, 1 µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GHSR.