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LearnRQ7252
antibody from NSJ Bioreagents
Targeting: SEPTIN9
AF17q25, KIAA0991, MSF, MSF1, PNUTL4, SEPT9, SeptD1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- RQ7252 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SEPT9 Antibody / Septin 9
- Antibody type
- Polyclonal
- Description
- This highly specific 45909 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SEPT9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) MOLT4, 3) HepG2, 4) A549, 5) Daudi, 6) RT4, 7) SH-SY5Y and 8) PC-3 cell lysate with SEPT9 antibody. Predicted molecular weight: 48-65 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human breast cancer tissue with SEPT9 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human liver cancer tissue with SEPT9 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with SEPT9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with SEPT9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with SEPT9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse lymph tissue with SEPT9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human U-251 cells with SEPT9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEPT9 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HeLa cells with SEPT9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEPT9 antibody.
