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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-88398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPYL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSGLDGVKRT TPLQTHSIII SDQVPSDQDA HQYLRLRDQS EATQVMAEPG EGGSETVALP PPPPSEEGGV PQDAAGRGGT PQIRVVGGRG HVAIKAGQEE GQPPAEGLAA ASVVMAADRS LKKGVQGGEK ALEICGAQRS ASELTAGAEA EAEEVKTGKC ATVSAAVAER ESAEVVKEGL AEKEVMEEQM EVEEQPPEGE EIEVAEEDRL EEEAREEEGP WPLHEALRMD; Positive Samples: U-87MG, SKOV3, LO2, HeLa, 293T, A-549; Cellular Location: Nucleus, nucleolus
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 4.31 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TSPYL1 in extracts of various cell lines. Samples were incubated with TSPYL1 Polyclonal antibody (Product # PA5-88398) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TSPYL1 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg TSPYL1 Polyclonal antibody (Product # PA5-88398). Western blot was performed from the immunoprecipitate using TSPYL1 Polyclonal antibody (Product # PA5-88398) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPYL1 in paraffin-embedded human liver cancer. Samples were incubated with TSPYL1 Polyclonal antibody (Product # PA5-88398) using a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TSPYL1 was performed in 200 µg extracts of HeLa cells using TSPYL1 Polyclonal Antibody (Product # PA5-88398). Western blot was performed from the immunoprecipitate using TSPYL1 Polyclonal Antibody.
