Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22056 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPYL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TSPYL1 Polyclonal Antibody (Product # PA5-22056). Sample (30 µg of whole cell lysate). Lane A: Molt-4. Lane B: Raji. 10% SDS PAGE. TSPYL1 Polyclonal Antibody (Product # PA5-22056) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TSPYL1 in paraformaldehyde-fixed HeLa cells using a TSPYL1 polyclonal antibody (Product # PA5-22056) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TSPYL1 antibody (Product # PA5-22056) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TSPYL1 was performed in paraffin-embedded human breast cancer tissue using TSPYL1 Polyclonal Antibody (Product # PA5-22056) at a dilution of 1:500.