GTX128065

antibody from GeneTex

Targeting: ATG3 APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96

Antibody data

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Validation data

ATG3 protein structure - GTX128065 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody (GTX128065) diluted at 1:1000.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41346

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG3 antibody detects ATG3 protein by Western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extract10 % SDS-PAGEATG3 antibody (GTX128065) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG3 antibody detects ATG3 protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract10 % SDS-PAGEATG3 antibody (GTX128065) dilution: 1:2000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG3 antibody detects ATG3 protein by Western blot analysis.A. 30 ?g C8D30 whole cell lysate/extractB. 30 ?g NIH-3T3 whole cell lysate/extractC. 30 ?g BCL-1 whole cell lysate/extractD. 30 ?g Raw264.7 whole cell lysate/extract10 % SDS-PAGEATG3 antibody (GTX128065) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG3 antibody detects ATG3 protein by Western blot analysis.A. 30 ?g K562 whole cell lysate/extractB. 30 ?g THP-1 whole cell lysate/extractC. 30 ?g HL-60 whole cell lysate/extract10 % SDS-PAGEATG3 antibody (GTX128065) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody (GTX128065) diluted at 1:1000.