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LearnPAB0740
antibody from Abnova Corporation
Targeting: ATG3
APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB0740 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0740, RRID:AB_1112903
- Product name
- ATG3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG3.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot under reducing conditions on 293 cell lysate using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 50.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody (Cat # PAB0740) .
- Validation comment
- Immunofluorescence
