PA5-17018

antibody from Invitrogen Antibodies

Targeting: ATG3 APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96

Provider product page for PA5-17018

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Other assay [2]

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Product number: PA5-17018 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATG3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: It is not recommended to aliquot this antibody.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Xenopus
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 29 μg/mL
Storage: -20°C

ATG3 protein structure - PA5-17018 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. miR-495 modulates autophagy. (A) RT-qPCR analysis was performed to determine the miR-495 expression levels in CAL-62 and SW579 cells transfected with sh-NC, sh-LGMN-1 or sh-LGMN-2. ***P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. Abolished ATG3 protein expression but comparable LC3-PE lipidation in thioglycolate-elicited atg7 -/- macrophages. Representative immunoblotting of LC3, ATG5, ATG7, and ss-actin in whole-cell lysates isolated from thioglycolate-elicited WT, ( Ai ) atg5 -/- , and ( Aii ) atg7 -/- peritoneal macrophages. Each lane represents a macrophage sample from an individual mouse. ( Aiii ) Quantification of LC3-I and LC3-II in atg5 -/- , atg7 -/- , and corresponding control macrophages normalized to ACTB/beta-actin (n = 6). Statistics were calculated using Student's t-test (two-tailed) with Welch correction for unequal variances; ** p < 0.01, *** p < 0.001. LC3 protein abundance in cellular fractions of WT, ( Bi ) atg5 -/- , and ( Bii ) atg7 -/- thioglycolate-elicited peritoneal macrophages with GAPDH as loading control. Each lane represents a sample pool from three mice. ( C ) mRNA expression of Atg5, Atg7, Atg3 , and Atg10 in WT, atg5 -/- , and atg7 -/- macrophages relative to Hprt (n = 12). ( Ci ) Representative immunoblotting of (ATG12 - )ATG5, ATG3 (upper blot), and ATG10 (lower blot), using the same samples obtained from three individual mice. ( Dii ) Protein quantification relative to ACTB/beta-actin (n = 5-6). Data represent mean values + SD. Statistics in ( C and Dii ) were calculated using one-way ANOVA with Bonferroni correction; * relative to WT; # atg5 -/- relative to atg7 -/- ; * , # p < 0.05, ** p < 0.01, *** /### p < 0.001. (E) Schematic presentation of the summa