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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF5450 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat ATG3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat ATG3 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9NT62- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Escitalopram oxalate induces apoptosis in U-87MG cells and autophagy in GBM8401 cells.
Chen VC, Hsieh YH, Chen LJ, Hsu TC, Tzang BS
Journal of cellular and molecular medicine 2018 Feb;22(2):1167-1178
Journal of cellular and molecular medicine 2018 Feb;22(2):1167-1178
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat ATG3 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ATG3 in RAW 264.7 Mouse Cell Line. ATG3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line, untreated (lower panel) or treated with LPS (upper panel), using Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
