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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- GTX630562 - Provider product page
- Provider
- GeneTex
- Product name
- COPD antibody [GT1318]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody [GT1318] (GTX630562) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COPD antibody [GT1318] detects COPD protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract |D. 30 £gg HepG2 whole cell lysate/extract7.5 % SDS-PAGECOPD antibody [GT1318] (GTX630562) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COPD antibody [GT1318] detects COPD protein by western blot analysis.A. 30 £gg Neuro2A whole cell lysate/extractB. 30 £gg GL261 whole cell lysate/extract C. 30 £gg C8D30 whole cell lysate/extract D. 30 £gg NIH-3T3 whole cell lysate/extract E. 30 £gg BCL-1 whole cell lysate/extract F. 30 £gg Raw 264.7 whole cell lysate/extract G. 30 £gg C2Cl2 whole cell lysate/extract7.5 % SDS-PAGECOPD antibody [GT1318] (GTX630562) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COPD antibody [GT1318] detects COPD protein by western blot analysis.A. 30 £gg PC-12 whole cell lysate/extract B. 30 £gg Rat2 whole cell lysate/extract7.5 % SDS-PAGECOPD antibody [GT1318] (GTX630562) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody [GT1318] (GTX630562) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COPD antibody [GT1318] detects COPD protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: COPD stained by COPD antibody [GT1318] (GTX630562) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.
