CF504315

antibody from Invitrogen Antibodies

Targeting: ARCN1 COPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF504315

Provider

Invitrogen Antibodies

Product name

ARCN1 Monoclonal Antibody (OTI6A6), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI6A6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARCN1 (RC210778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ARCN1. Positive lysates LY400624 (100 µg) and LC400624 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARCN1 (RC210778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ARCN1. Positive lysates LY400624 (100 µg) and LC400624 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-ARCN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-ARCN1 mouse monoclonal antibody (TA504315) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARCN1(RC210778).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504315)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504315)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504315)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504315)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either RC210778 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARCN1 antibody (TA504315), and then analyzed by flow cytometry.