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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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- Product number
- PA5-21484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COPD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (82%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A weak COPI binding motif in the cytoplasmic tail of SARS-CoV-2 spike glycoprotein is necessary for its cleavage, glycosylation, and localization.
Jennings BC, Kornfeld S, Doray B
FEBS letters 2021 Jul;595(13):1758-1767
FEBS letters 2021 Jul;595(13):1758-1767
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COPD Polyclonal Antibody (Product # PA5-21484). Sample (30 µg whole cell lysate). A: H1299. B: Hep G2. 7.5% SDS PAGE. COPD Polyclonal Antibody (Product # PA5-21484) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COPD in paraformaldehyde-fixed HeLa cells using a COPD polyclonal antibody (Product # PA5-21484) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (red) at a 1:2500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD (Product # PA5-21484) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (red) at 1:2,500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD (Product # PA5-21484) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (red) at 1:2,500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of COPD was performed in frozen-sectioned mouse mouse cerebellum tissue using COPD Polyclonal Antibody (Product # PA5-21484) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of COPD was performed in paraffin-embedded mouse brain tissue using COPD Polyclonal Antibody (Product # PA5-21484) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of COPD was performed in frozen-sectioned mouse mouse cerebellum tissue using COPD Polyclonal Antibody (Product # PA5-21484) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
