BS2540

antibody from Bioworld Technology, Inc

Targeting: MGMT

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS2540

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#BS2540, RRID:AB_1662884

Product name

MGMT (C5) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to the N-terminus of Human MGMT.

Description

MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.