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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- UM800006 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800006, RRID:AB_2629123
- Product name
- MGMT mouse monoclonal antibody,clone UMAB52
- Antibody type
- Monoclonal
- Description
- MGMT mouse monoclonal antibody,clone UMAB52
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MGMT
- Isotype
- IgG
- Antibody clone number
- UMAB52
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Thioridazine inhibits autophagy and sensitizes glioblastoma cells to temozolomide.
Johannessen TC, Hasan-Olive MM, Zhu H, Denisova O, Grudic A, Latif MA, Saed H, Varughese JK, Røsland GV, Yang N, Sundstrøm T, Nordal A, Tronstad KJ, Wang J, Lund-Johansen M, Simonsen A, Janji B, Westermarck J, Bjerkvig R, Prestegarden L
International journal of cancer 2019 Apr 1;144(7):1735-1745
International journal of cancer 2019 Apr 1;144(7):1735-1745
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MGMT monoclonal antibody (Clone UMAB52).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using MGMT mouse monoclonal antibody (UM800006, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-MGMT mouse monoclonal antibody (UM800006, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
