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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB3299 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse MGMT Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse MGMT in Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P26187- Isotype
- IgG
- Antibody clone number
- 300008
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.
Rajesh P, Rajesh C, Wyatt MD, Pittman DL
DNA repair 2010 Apr 4;9(4):458-67
DNA repair 2010 Apr 4;9(4):458-67
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse MGMT by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse MGMT Monoclonal Antibody (Catalog # MAB3299) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MGMT at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
