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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab115239 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- MGMT Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on.ApplicationWB: 0.5-3µg/mLIHC: 5-30µg/mLProtein FunctionInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-207- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MGMT Antibody (Ab115239) - Western Blot All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: 2.6 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MGMT Antibody (Ab115239) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL MGMT Antibody (Ab115239) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
