601902
antibody from BioLegend
Targeting: EHMT1
bA188C12.1, EHMT1-IT1, Eu-HMTase1, FLJ12879, FLJ40292, KIAA1876, KMT1D
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 601902 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#601902, RRID:AB_315595
- Product name
- Purified anti-Histone H3
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial), N-terminal
- Description
- Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. The Poly6019 antibody has been shown to be useful for Western blotting of human and mouse histone H3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Murine cytomegalovirus targets transcription factor ATF4 to exploit the unfolded-protein response.
Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth.
Qian Z, Xuan B, Chapa TJ, Gualberto N, Yu D
Journal of virology 2012 Jun;86(12):6712-23
Journal of virology 2012 Jun;86(12):6712-23
Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth.
Peng DJ, Zeng M, Muromoto R, Matsuda T, Shimoda K, Subramaniam M, Spelsberg TC, Wei WZ, Venuprasad K
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5638-47
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5638-47
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-histone H3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated