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LearnPA5-31160
antibody from Invitrogen Antibodies
Targeting: GLRX3
bA500G10.4, GLRX4, GRX3, GRX4, PICOT, TXNL2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-31160 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TXNL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (80%), Pig (94%), Chicken (86%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.
Zhou H, Qu Z, Mossine VV, Nknolise DL, Li J, Chen Z, Cheng J, Greenlief CM, Mawhinney TP, Brown PN, Fritsche KL, Hannink M, Lubahn DB, Sun GY, Gu Z
PloS one 2014;9(11):e113531
PloS one 2014;9(11):e113531
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TXNL2 Polyclonal Antibody (Product # PA5-31160). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. TXNL2 Polyclonal Antibody (Product # PA5-31160) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TXNL2 Polyclonal Antibody (Product # PA5-31160). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. TXNL2 Polyclonal Antibody (Product # PA5-31160) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TXNL2 in methanol-fixed A431 cells using a TXNL2 polyclonal antibody (Product # PA5-31160) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Validation of expression profiling of proteins by Western blotting. Four of the identified proteins, PRDX1 ( A ), GLRX3 ( B ), ENO1 ( C ), and CASP1 ( D ), responding to AGE and/or FruArg treatment in LPS-stimulated BV-2 cells were validated using Western blotting. Protein intensities were quantified by Image J software, normalized to beta-actin, and expressed as percentage of untreated controls. Data are means +- SEM (n>=5); #, P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TXNL2 was performed in 293T whole cell extracts using 5 µg of TXNL2 Polyclonal Antibody (Product # PA5-31160). Samples were transferred to a membrane and probed with TXNL2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
