Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-77296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CACNA1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.
Okada T, Kato D, Nomura Y, Obata N, Quan X, Morinaga A, Yano H, Guo Z, Aoyama Y, Tachibana Y, Moorhouse AJ, Matoba O, Takiguchi T, Mizobuchi S, Wake H
Science advances 2021 Mar;7(12)
Science advances 2021 Mar;7(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes with CaV2.2 polyclonal antibody (Product # PA5-77296) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CaV2.2 in paraformaldehyde-fixed and permeabilized rat dorsal root ganglion. A) Samples were probed with CaV2.2 polyclonal antibody (Product # PA5-77296) at a dilution of 1:200, and incubated with goat anti-rabbit-AlexaFluor-555 and Hoechst. B) Nuclei stained image. C) Merged images of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CaV2.2 in mouse cerebellum. A) Samples were probed with CaV2.2 polyclonal antibody (Product # PA5-77296) using a dilution of 1:100, and incubated with calbindin (green) (red). B) Stained Purkinje cell image (green). C) Merged image of Panels A and B.