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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB3387 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3387, RRID:AB_1580239
- Product name
- ROS1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ROS1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Isolation and characterization of the human DTK receptor tyrosine kinase.
Crosier KE, Hall LR, Lewis PM, Morris CM, Wood CR, Morris JC, Crosier PS
Growth factors (Chur, Switzerland) 1994;11(2):137-44
Growth factors (Chur, Switzerland) 1994;11(2):137-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ROS1 polyclonal antibody (Cat # PAB3387) in HL-60 cell lysate. ROS (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- A : Negative control of HeLa cells without FITC-conjugated goat anti-rabbit lgG (H+L). FITC-conjugated goat anti-rabbit lgG (H+L) emits green fluorescence.B : Immunofluorescence analysis of ROS1 polyclonal antibody (Cat # PAB3387) in HeLa cells.0.025 mg/mL primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (H+L).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ROS1 polyclonal antibody (Cat # PAB3387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
