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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-26760 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROS1 Monoclonal Antibody (OTI1A1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1A1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ROS1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ROS1 (Product # MA5-26760) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ROS1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ROS1 (Product # MA5-26760) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ROS1 in HeLa, HCC78 cells using 35 µg per lane. Samples were probed with ROS1 (Product # MA5-26760) monoclonal antibody at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ROS1 Monoclonal Antibody (OTI1A1) (Product # MA5-26760) and a ~260kDa band corresponding to Proto-oncogene tyrosine-protein kinase ROS was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), A549 (Lane 2), U-87 MG (Lane 3), HeLa (Lane 4), BeWo (Lane 5) and MCF7 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ROS1 in HCC78 cells. Cells were transfected with a plasmid overexpressing ROS1 and probed with a ROS1 monoclonal antibody (Product # MA5-26760). HeLa cells were used as a negative control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded HCC78 (left) and HeLa (right) xenograft tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a ROS1 monoclonal antibody (Product # MA5-26760) at a dilution of 0.2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded non-small cell lung cancer tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a ROS1 monoclonal antibody (Product # MA5-26760) at a dilution of 0.2 µg/mL.
