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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
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- Product number
- PA5-27655 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HYOU1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7, MDA-MB-231, mouse liver, rat brain. Predicted reactivity: Mouse (92%), Rat (90%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references ERp18 regulates activation of ATF6α during unfolded protein response.
Oka OB, van Lith M, Rudolf J, Tungkum W, Pringle MA, Bulleid NJ
The EMBO journal 2019 Aug 1;38(15):e100990
The EMBO journal 2019 Aug 1;38(15):e100990
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HYOU1 in methanol-fixed Hep3B cells using a HYOU1 polyclonal antibody (Product # PA5-27655) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HYOU1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HYOU1 Polyclonal Antibody (Product # PA5-27655) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HYOU1 was performed in U2OS cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HYOU1 Polyclonal Antibody (Product # PA5-27655) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
