Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF611 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse EphB6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse EphB6 in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) EphB2, rmEphB3, and rmEphB4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O08644
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells.
EPHB6 and testosterone in concert regulate epinephrine release by adrenal gland chromaffin cells.
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Shi W, Wang Y, Peng J, Qi S, Vitale N, Kaneda N, Murata T, Luo H, Wu J
The Journal of biological chemistry 2019 Apr 26;294(17):6871-6887
The Journal of biological chemistry 2019 Apr 26;294(17):6871-6887
EPHB6 and testosterone in concert regulate epinephrine release by adrenal gland chromaffin cells.
Wang Y, Shi W, Blanchette A, Peng J, Qi S, Luo H, Ledoux J, Wu J
Scientific reports 2018 Jan 16;8(1):842
Scientific reports 2018 Jan 16;8(1):842
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
European journal of immunology 2012 Jun;42(6):1562-72
European journal of immunology 2012 Jun;42(6):1562-72
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature 2011 May 19;473(7347):372-5
Nature 2011 May 19;473(7347):372-5
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse EphB6 by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF611) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB6 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.