Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PAB2074 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2074, RRID:AB_1574725
- Product name
- EPHB6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHB6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The EphB6 receptor inhibits JNK activation in T lymphocytes and modulates T cell receptor-mediated responses.
EphB6 crosslinking results in costimulation of T cells.
Multiple roles of EPH receptors and ephrins in neural development.
Freywald A, Sharfe N, Rashotte C, Grunberger T, Roifman CM
The Journal of biological chemistry 2003 Mar 21;278(12):10150-6
The Journal of biological chemistry 2003 Mar 21;278(12):10150-6
EphB6 crosslinking results in costimulation of T cells.
Luo H, Yu G, Wu Y, Wu J
The Journal of clinical investigation 2002 Oct;110(8):1141-50
The Journal of clinical investigation 2002 Oct;110(8):1141-50
Multiple roles of EPH receptors and ephrins in neural development.
Wilkinson DG
Nature reviews. Neuroscience 2001 Mar;2(3):155-64
Nature reviews. Neuroscience 2001 Mar;2(3):155-64
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB6 polyclonal antibody (Cat # PAB2074) in A-549 cell lysate. EPHB6 (arrow) was detected using purified Polyclonal antibody.Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB6 polyclonal antibody (Cat # PAB2074) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using EPHB6 polyclonal antibody (Cat # PAB2074)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry