GTX103247

antibody from GeneTex

Targeting: AMPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX103247

Provider

GeneTex

Proper citation

GeneTex Cat#GTX103247, RRID:AB_1949643

Product name

Amphiphysin antibody [N1N2], N-term

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.A. 50 ?g mouse brain lysate/extract 7.5 % SDS-PAGEAmphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.A. 50 ?g rat brain lysate/extract 5 % SDS-PAGEAmphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects Amphiphysin protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Amphiphysin stained by Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin(GTX103247) antibody at 1:100 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.