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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- PA5-144631 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive control: SW480 cell lysate, SK-Br-3 cell lysate, HepG2, HT-29, rat testis tissue, human kidney tissue, mouse colon tissue, human placenta tissue. Predicted band size: 87 kDa Subcellular Location: Cell membrane, Membrane.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRPV6 in different various lysates. Lane 1: SW480 cell lysate; Lane 2: SK-Br-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa. Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6)Exposure time: 30 seconds; 8% SDS-PAGE gel. Primary antibody TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TRPV6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were incubated with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Followed by secondary antibody Alexa Fluor®488 Goat anti-Rabbit IgG at a dilution of 1:100. The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TRPV6 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were incubated with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Followed by secondary antibody Alexa Fluor®488 Goat anti-Rabbit IgG at a dilution of 1:100. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPV6 in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPV6 in paraffin-embedded rat testis tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPV6 in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPV6 in paraffin-embedded mouse colon tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRPV6 in paraffin-embedded mouse colon tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with TRPV6 polyclonal antibody (Product # PA5-144631) with a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TRPV6 in HT-29 cells. The cells were fixed, permeabilized, and then stained with TRPV6 polyclonal antibody (Product # PA5-144631) at a dilution of 1:100 (red). After incubation of the primary antibody at room temperature for an hour, the cells were then stained with secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG at a dilution of 1:500 for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
