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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA328743 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-TRPV5
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-TRPV5
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TRPV5
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates: 1,2. Anti-TRPV5 antibody, (1:200). 3,4. Anti-TRPV5 antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPV5 in PC3 cells. Immunocytochemical staining of paraformaldehyde-fixed PC-3 cells with Anti-TRPV5 antibody, (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Same as in (A) but pre-treated with TRPV5 peptide antigen (1:20). Hoechst 33342 is used as the counterstain (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPV5 in rat kidney. Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 antibody, (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.
- Validation comment
- IHC
