PA5-109274

antibody from Invitrogen Antibodies

Targeting: GPX4 MCSP, PHGPx

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109274

Provider

Invitrogen Antibodies

Product name

GPX4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX4 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GPX4 polyclonal antibody (Product # PA5-109274), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GPX4 in paraffin-embedded mouse testis tissue. Samples were incubated with GPX4 polyclonal antibody (Product # PA5-109274), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GPX4 in HepG2 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with GPX4 polyclonal antibody (Product # PA5-109274) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GPX4 in HepG2 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with GPX4 polyclonal antibody (Product # PA5-109274) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.