PA5-19710

antibody from Invitrogen Antibodies

Targeting: GPX4 MCSP, PHGPx

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19710

Provider

Invitrogen Antibodies

Product name

GPX4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with many species based on sequence homology.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human Heart Tissue Lysate using Product # PA5-19710, Glutathione Peroxidase 4 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Human Skeletal Muscle Tissue Lysate at a dilution of 1 µg/mL (Lane 2). Staining of Human Colon Tissue Lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GPX4 Polyclonal Antibody (Product # PA5-19710) and a ~19.5 kDa band was observed across the panel tested. Tissue lysates (30 µg lysate) of Mouse Testis (Lane 1), Rat Testis (Lane 2), Mouse Liver (Lane 3), whole cell extracts (30 µg lysate) of MCF-7 (Lane 4), HeLa (Lane 5) and Daudi (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using Product # PA5-19710, anti-Glutathione Peroxidase antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with TBS-T (20mins), BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).