LS-C820648

antibody from LSBio

Targeting: COX5B

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C820648

Provider

LSBio

Product name

COX5B Antibody (clone 020, Preservative Free) LS-C820648

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

020

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-COX5B rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 15 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

COX5B was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C:0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-COX5B rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-COX5B rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

COX5B was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C:0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-COX5B rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-COX5B rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa.