Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX5B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASGGGVPTDE EQATGLEREI MLAAKKGLDP YNVLAPKGAS GTREDPNLVP SISNKRIVGC ICEEDNTSVV WFWLHKGEAQ RCPRCGAHYK LVPQQLAH; Positive Samples: HepG2, 22Rv1, SW480, HeLa, A-549, Mouse heart, Mouse kidney, Rat heart; Cellular Location: Mitochondrion inner membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.68 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references First identification of ITM2B interactome in the human retina.
Wohlschlegel J, Argentini M, Michiels C, Letellier C, Forster V, Condroyer C, He Z, Thuret G, Zeitz C, Léger T, Audo I
Scientific reports 2021 Aug 26;11(1):17210
Scientific reports 2021 Aug 26;11(1):17210
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX5B in extracts of various cell lines using COX5B Polyclonal Antibody (Product # PA5-96189) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in C6 cells using COX5B Polyclonal Antibody (Product # PA5-96189).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in NIH-3T3 cells using COX5B Polyclonal Antibody (Product # PA5-96189) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in U-2 OS cells using COX5B Polyclonal Antibody (Product # PA5-96189) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of COX5B was performed in 200 µg extracts of HepG2 cells using COX5B Polyclonal Antibody (Product # PA5-96189). Western blot was performed from the immunoprecipitate using COX5B Polyclonal Antibody.
