PA5-96189

antibody from Invitrogen Antibodies

Targeting: COX5B

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96189

Provider

Invitrogen Antibodies

Product name

COX5B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ASGGGVPTDE EQATGLEREI MLAAKKGLDP YNVLAPKGAS GTREDPNLVP SISNKRIVGC ICEEDNTSVV WFWLHKGEAQ RCPRCGAHYK LVPQQLAH; Positive Samples: HepG2, 22Rv1, SW480, HeLa, A-549, Mouse heart, Mouse kidney, Rat heart; Cellular Location: Mitochondrion inner membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.68 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

First identification of ITM2B interactome in the human retina.

Wohlschlegel J, Argentini M, Michiels C, Letellier C, Forster V, Condroyer C, He Z, Thuret G, Zeitz C, Léger T, Audo I
Scientific reports 2021 Aug 26;11(1):17210

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in C6 cells using COX5B Polyclonal Antibody (Product # PA5-96189).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in NIH-3T3 cells using COX5B Polyclonal Antibody (Product # PA5-96189) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX5B was performed in U-2 OS cells using COX5B Polyclonal Antibody (Product # PA5-96189) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COX5B in U-2 OS cells. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COX5B in C6 cells. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COX5B in NIH-3T3 cells. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of COX5B in 200 μg extracts of HepG2 cells. Samples were precipitated with 3 μg COX5B Polyclonal antibody (Product # PA5-96189). Western blot was performed from the immunoprecipitate using COX5B Polyclonal antibody (Product # PA5-96189) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COX5B in paraffin-embedded rat kidney. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COX5B in paraffin-embedded human colon. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COX5B in paraffin-embedded mouse kidney. Samples were incubated with COX5B Polyclonal antibody (Product # PA5-96189) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of COX5B was performed in 200 µg extracts of HepG2 cells using COX5B Polyclonal Antibody (Product # PA5-96189). Western blot was performed from the immunoprecipitate using COX5B Polyclonal Antibody.