- 459110 - Invitrogen Antibodies COX5B antibody

Submitted references GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function.

Timper K, Del Río-Martín A, Cremer AL, Bremser S, Alber J, Giavalisco P, Varela L, Heilinger C, Nolte H, Trifunovic A, Horvath TL, Kloppenburg P, Backes H, Brüning JC
Cell metabolism 2020 Jun 2;31(6):1189-1205.e13

Mild Impairment of Mitochondrial OXPHOS Promotes Fatty Acid Utilization in POMC Neurons and Improves Glucose Homeostasis in Obesity.

Timper K, Paeger L, Sánchez-Lasheras C, Varela L, Jais A, Nolte H, Vogt MC, Hausen AC, Heilinger C, Evers N, Pospisilik JA, Penninger JM, Taylor EB, Horvath TL, Kloppenburg P, Brüning JC
Cell reports 2018 Oct 9;25(2):383-397.e10

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.

Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology 2017 Mar 6;216(3):675-693

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Korwitz A, Merkwirth C, Richter-Dennerlein R, Tröder SE, Sprenger HG, Quirós PM, López-Otín C, Rugarli EI, Langer T
The Journal of cell biology 2016 Jan 18;212(2):157-66

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of COX5B in heart mitochondria extracts using a COX5B Monoclonal antibody (Product # 459110) at a concentration of 1 µg/mL. Lane 1 : Human heart mitochondria at 5 µg, Lane 2 : Bovine heart mitochondria at 1 µg, Lane 3 : Rat heart mitochondria at 10 µg, Lane 4 : Mouse heart mitochondria at 10 µg. Predicted band size: 14 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of COX5B in HepG2 cells using a COX5B Monoclonal Antibody (Product # 459110) at 1 µg/1x10^6 cells, shown in red. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) at a 1:500 dilution. Isotype control, as seen in black, was a mouse IgG2b antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Liver-specific Cluh deletion affects mitochondrial distribution and structure, assembled respiratory supercomplexes, and respiratory capacity. (A) Representative confocal images of livers of 8-wk-old mice of the indicated genotypes. To analyze mitochondrial morphology, mice were crossed with a stop-mito-YFP reporter line activated by Cre recombination. n = 4. Bars, 5 um. (B) Oxygen consumption of mitochondria isolated from livers of 8-wk-old mice. State III respiration was measured in the presence of pyruvate, malate, glutamate, and ADP (complex I [CI]), followed by addition of succinate (complex I + complex II [CI + CII]). The proton leak was measured after addition of oligomycin, whereas maximal respiration was assessed by CCCP titration. n = 5. Graph shows means +- SEM. **, P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. Cluh -deficient MEFs mimic liver phenotypes. (A) Oxygen consumption of intact MEFs cultured in glucose or galactose medium. The proton leak was measured after the addition of oligomycin, whereas maximal respiration was assessed by CCCP titration. n >= 4. (B) Mitochondrial morphology in MEFs transfected with mito-mCherry. Graphs show the mean aspect ratio (area/perimeter) on the x axis and the number of mitochondria on the y axis for individual cells from three independent experiments. Right panels show representative images of mitochondrial morphology at the indicated time points. Bar, 12 um. (C) Growth curves of MEFs cultured in glucose or galactose medium during five consecutive days. n = 3. (D) Representative images of LD staining in MEFs grown in glucose medium. Nuclei were stained with DAPI (blue), and LDs were stained with BODIPY 493/503 (green). Bar, 20 um. (E) Quantification of LD staining shown in D. 50 cells were analyzed per genotype per experiment. Graph shows the mean area of LDs per cell. n = 3. (A, C, and E) Error bars are means +- SEM. *, P

459110

Antibody data