R20331

antibody from NSJ Bioreagents

Targeting: RBFOX3 FOX-3, HRNBP3, NeuN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R20331

Provider

NSJ Bioreagents

Product name

Recombinant NeuN Antibody / Fox3 / N-Terminal

Antibody type

Monoclonal

Description

This highly specific Recombinant NeuN antibody is suitable for use in Western blot/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM312

Vial size

100 ul

Concentration

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant NeuN antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain lysate with recombinant NeuN antibody at 1:1000 dilution. Expected molecular weight: 46-48 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain lysate with recombinant NeuN antibody at 1:1000 dilution. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant NeuN antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant NeuN antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant NeuN antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin fixed and paraffin embedded mouse brain tissue with recombinant NeuN antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.