LS-C682388

antibody from LSBio

Targeting: RBFOX3 FOX-3, HRNBP3, NeuN

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C682388

Provider

LSBio

Product name

RBFOX3 / NEUN Antibody (aa1-140, clone 5A4B9) LS-C682388

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5A4B9

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RBFOX3 mAb against human *** (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher