NBP1-77686

antibody from Novus Biologicals

Targeting: RBFOX3 FOX-3, HRNBP3, NeuN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-77686

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-77686, RRID:AB_11009597

Product name

Rabbit Polyclonal RBFOX3/NeuN Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.08 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Taurine/Pilocarpine Interaction in the Malnourished Rat Brain: A Behavioral, Electrophysiological, and Immunohistochemical Analysis.

Francisco EDS, Mendes-da-Silva RF, de Castro CBL, Soares GSF, Guedes RCA
Frontiers in neuroscience 2019;13:981

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Delayed Azithromycin Treatment Improves Recovery After Mouse Spinal Cord Injury.

Kopper TJ, McFarlane KE, Bailey WM, Orr MB, Zhang B, Gensel JC
Frontiers in cellular neuroscience 2019;13:490

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Low-dose irradiated mesenchymal stromal cells break tumor defensive properties in vivo.

Stefani FR, Eberstål S, Vergani S, Kristiansen TA, Bengzon J
International journal of cancer 2018 Nov 1;143(9):2200-2212

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A deficiency of the GluN2C subunit of the N-methyl-D-aspartate receptor is neuroprotective in a mouse model of ischemic stroke.

Holmes A, Zhou N, Donahue DL, Balsara R, Castellino FJ
Biochemical and biophysical research communications 2018 Jan 1;495(1):136-144

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Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice.

DeMars KM, Pacheco SC, Yang C, Siwarski DM, Candelario-Jalil E
Frontiers in neurology 2017;8:363

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Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury.

Zhang B, Bailey WM, McVicar AL, Gensel JC
Neurobiology of aging 2016 Nov;47:157-167

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Pathogenic Upregulation of Glial Lipocalin-2 in the Parkinsonian Dopaminergic System.

Kim BW, Jeong KH, Kim JH, Jin M, Kim JH, Lee MG, Choi DK, Won SY, McLean C, Jeon MT, Lee HW, Kim SR, Suk K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 May 18;36(20):5608-22

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The effect of constitutive over-expression of insulin-like growth factor 1 on the cognitive function in aged mice.

Hu A, Yuan H, Wu L, Chen R, Chen Q, Zhang T, Wang Z, Liu P, Zhu X
Brain research 2016 Jan 15;1631:204-13

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Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells.

Robinson M, Chapani P, Styan T, Vaidyanathan R, Willerth SM
Stem cell reviews and reports 2016 Aug;12(4):476-83

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Spinal NF-κB and chemokine ligand 5 expression during spinal glial cell activation in a neuropathic pain model.

Yin Q, Fan Q, Zhao Y, Cheng MY, Liu H, Li J, Lu FF, Jia JT, Cheng W, Yan CD
PloS one 2015;10(1):e0115120

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Double minute amplification of mutant PDGF receptor α in a mouse glioma model.

Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova NM, Jahanshahi M, Olson LE, Soriano P, Friedel RH
Scientific reports 2015 Feb 16;5:8468

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A study of the potential neuroprotective effect of riluzole on locomotor networks of the neonatal rat spinal cord in vitro damaged by excitotoxicity.

Sámano C, Nasrabady SE, Nistri A
Neuroscience 2012 Oct 11;222:356-65

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBFOX3/NeuN Antibody [NBP1-77686] - Western blot image submitted by a verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - JAK3 colocalized with neurons labeled with NeuN. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fneur.2017.00363/full), licensed under a CC-BY licence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on Hek293 cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.