PA5-81013

antibody from Invitrogen Antibodies

Targeting: SERPIND1 D22S673, HC-II, HC2, HCF2, HLS2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81013

Provider

Invitrogen Antibodies

Product name

Heparin Cofactor II Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human SerpinD1/HC2.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Heparin Cofactor II Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with Heparin Cofactor II Polyclonal Antibody (Product # PA5-81013) and 15 µL of 50 % Protein G agarose. Primary antibody: Heparin Cofactor II Polyclonal Antibody, at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 70 kDa.