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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA1-30290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-EphA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-30290 detects EphA1 from human samples. PA1-30290 has been successfully used in immunohistochemistry (paraffin), Western blot, and ELISA applications. The PA1-30290 immunogen is a synthetic peptide conjugated to KLH, corresponding to amino acids 44-60 of Human Eph A1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Eph Receptor A1 in HeLa cell lysate using an Eph Receptor A1 polyclonal antibody (Product # PA1-30290) followed by detection using an HRP-anti-Rabbit secondary antibody and chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Eph Receptor A1 in formalin-fixed, paraffin-embedded human cancer tissue using an Eph Receptor A1 polyclonal antibody (Product # PA1-30290) peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Eph Receptor A1 in HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) using an Eph Receptor A1 polyclonal antibody (Product # PA1-30290). Secondary detection was performed using a FITC-conjugated goat-anti-rabbit secondary antibody.
