AF638

antibody from Novus Biologicals

Targeting: EPHA1 EPH, EPHT, EPHT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF638

Provider

Novus Biologicals

Product name

Goat Polyclonal EphA1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human EphA1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA8, rmEphA4, recombinant rat (rr) EphA5, rmEphA2, rmEphA6, and rmEphA7 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis.

Gordon K, Kochkodan JJ, Blatt H, Lin SY, Kaplan N, Johnston A, Swindell WR, Hoover P, Schlosser BJ, Elder JT, Gudjonsson JE, Getsios S
The Journal of investigative dermatology 2013 Mar;133(3):712-722

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human EphA1 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphA1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.