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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB30341 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse EphA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse EphA1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse EphA2, recombinant human (rh) EphA3, or rhEphA5 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
AAH71215- Isotype
- IgG
- Antibody clone number
- 404517
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- EphA1 in D3 Mouse Cell Line. EphA1 was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse EphA1 Monoclonal Antibody (Catalog # MAB30341) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
