HPA028136

antibody from Atlas Antibodies

Targeting: NASP DKFZp547F162, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, PRO1999

Immunocytochemistry

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: HPA028136 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA028136, RRID:AB_2672497
Product name: Anti-NASP
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human NASP, Gene description: nuclear autoantigenic sperm protein (histone-binding), Alternative Gene Names: DKFZp547F162, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, PRO1999, Validated applications: ICC, IHC, WB, Uniprot ID: P49321, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

NASP protein structure - HPA028136 shown in red.

Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting NASP or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting NASP Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:159
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s9281
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no 2: s9280
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s9282
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R27220
Appendix: 560d112b1ec4f.pdf

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NASP antibody. Remaining relative intensity is presented.
Sample type: Human
Protocol: Protocol

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and NASP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400884).
Sample type: Human
Protocol: Protocol