Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R20183 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgA1 Antibody
- Antibody type
- Recombinant
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM124
- Vial size
- 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgA1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to IgA. Very slightly cross reacts with IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.