R20183BTN

antibody from NSJ Bioreagents

Targeting: IGHA1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

R20183BTN

Provider

NSJ Bioreagents

Product name

Recombinant Human IgA1 Antibody

Antibody type

Recombinant

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Antibody clone number

RM124

Vial size

50 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgA1 antibody at -20°C.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.