M01548

GAA antibody from Boster Biological Technology

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunohistochemistry

Data presented on provider website

Flow cytometry

Data presented on provider website

Provider product page for M01548

Antibody Data

Product number

M01548

Provider

Boster Biological Technology

Product name

Anti-GAA Antibody Picoband™ (monoclonal, 2G7)

Provider product page

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for peroxiredoxin 6 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2G7

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of GAA using anti- GAAantibody (M01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- GAA antigen affinity purified polyclonal antibody (Catalog # M01548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.

Additional image