MA5-36228

antibody from Invitrogen Antibodies

Targeting: GAA

Antibody data

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Validation data

Product number: MA5-36228 - Provider product page
Provider: Invitrogen Antibodies
Product name: GAA Monoclonal Antibody (2G7)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human A549 tissue, human HEK293 whole cell, human PC-3 whole cell. IHC: human lung cancer tissue, human mammary cancer tissue, human prostatic cancer tissue. ICC/IF: A549 cell, A549 cell .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 2G7
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

GAA protein structure - MA5-36228 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.