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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-29051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LYAG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.76 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LYAG using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a LYAG polyclonal antibody (Product # PA5-29051) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LYAG was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LYAG Polyclonal Antibody (Product # PA5-29051) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of LYAG was performed in paraffin-embedded human breast carcinoma tissue using LYAG Polyclonal Antibody (Product # PA5-29051) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LYAG Polyclonal Antibody detects LYAG protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYAG stained by LYAG Polyclonal Antibody (Product # PA5-29051) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
